http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SU-1264037-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_21772d500360dfea521e67c208de394a |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
filingDate | 1983-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1986-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4bc4475e11aa6301a6ba376ae0139759 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd33803af917e7fa029884e082259ac4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffd1e5d5382618e514765094082dec05 |
publicationDate | 1986-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | SU-1264037-A1 |
titleOfInvention | Method for fixation of myocardium tissue |
abstract | The invention relates to the field of medicine, namely, cardiology, and can be used to study myocardial ultrastructure. And biochemical studies. The purpose of the invention is to prevent arterial catabolism of high energy phosphates in myocardial tissue. After the animal is put into anesthesia, the chest is quickly opened, into the left ventricular cavity in the apex of the ventricle through a needle with a channel diameter of 1-3 mm, dressed in a syringe, the fixation solution cooled to (-2) 0 ° C is inserted in a volume of 70 -75% of the total volume of the left ventricular cavity of the heart every second for 5-7 seconds to complete cardiac arrest. For a morphological study of the ultrastructure of cardiomyocytes, small pieces of tissue are fixed in 1% buffered solution from glutaraldehyde, dehydrated and closed according to the standard technique with AL, followed by a stereological analysis of the electron diffraction patterns. 2 tab. |
priorityDate | 1983-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.