| abstract |
The method of isolation of hyaluronidase. from the culture fluid of the pathogenic producer strain by precipitation of the enzyme with ethyl alcohol in a 1: 1 ratio, separation of the dissolution otgadka followed by chromatographic purification on a polysaccharide sorbent, characterized in that, in order to increase the purity of the target product, the culture fluid of the Staphy2OCOCCUS aPbus 0- strain is used 15, diethyl aminoethyl cellulose is used as a sorbent, and during chromatography sorption is carried out under conditions of 0.006 M phosphate buffer with a pH of 7.0-7.2, and elution is carried out with 0.06 M phosphate buffer under the same znacheO) or pH. | with joint venture til 4 |