| abstract |
Isolated nucleic acid (I) from coryneform bacteria which is at least 70% identical with a sequence that encodes a 145 amino acid (aa) polypeptide (II) fully defined in the specification, encodes a polypeptide at least 70% identical with (II), its complement or contains at least 15 consecutive bases of (I). Independent claims are also included for the following: (1) vector containing (I), deposited in Escherichia coli DH5 alpha /p DELTA rpk, as DSM 13158 (map reproduced in specification); (2) as host cells, coryneform bacteria that have a deletion or insertion in the rplk gene; and (3) method (M1) for producing L-aa, especially lysine, by fermenting bacteria in which at least the rplk gene has been weakened. |