| abstract |
Described is a hirudin precursor containing a signal sequence selected from the group comprising the signal sequence of the outer membrane protein of Serratia marcescens, the oprF protein of Pseudomonas fluorescens, the fumarate reductase of Shewanella putrifaciens, onto which there is a C-terminal attachment of the a sequence of Leu-hirudin, in which the hirudin precursor occurs as an intermediate, and use the hirudine precursor. |