http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SK-282589-B6
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fd03ff6c496cbf41f020f2ea07ba32bd |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D3-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-08 |
filingDate | 1998-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c680a66fc34cc3a58799281c4ca52476 |
publicationDate | 2002-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | SK-282589-B6 |
titleOfInvention | Method of fractionation of honey bee jelly and isolation of protein complex of bee royal jelly, pharmaceutical and cosmetic composition containing protein complex |
abstract | The present invention relates to a method for fractionating a honey bee jelly and isolating a protein complex having a molecular weight of from 49 to 66 kDa. The royal jelly or its mixture with water and / or glycerol is separated into individual fractions by ultracentrifugation at 20,000 to 300,000 x g, the gel fraction is mixed with water and the protein complex is recovered by repeated centrifugation as a sedimented gel with a water content of 60 to 80 wt.%, Which contains 96 to 98 wt. % basic structural protein with a molecular weight of 55 kDa and 2 - 4 wt. mixtures of proteins with molecular weights of 49, 55, 60 and 66 kDa with variable proportions. The royal jelly is divided into three fractions, liquid, gel and semi-solid, by ultracentrifugation at 150,000 to 300,000 x g; the royal jelly / water and / or glycerol mixture is divided into two fractions, liquid and gel, by ultracentrifugation at 150,000 to 300,000 x g, with ultracentrifugation at 250,000 x g for 40 to 200 minutes at a temperature not exceeding 15 ° C. |
priorityDate | 1998-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 767.