http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SK-280028-B6
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6462 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21073 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 |
| filingDate | 1990-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1cc9ea188785140fa438469c60f24ae2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec0ec66d5b439b158d9f4cb6de10bde5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b863028eb1cda20da692d73ebf685db3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c415d2b9a288fe3e4c555eee3095b25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a7d9e1c48e4e52eca5bf3b2c64dffa5e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7fa5f865f3dba7f850603f5f4c5106d5 |
| publicationDate | 1999-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SK-280028-B6 |
| titleOfInvention | PLASMIDES, METHODS OF PRODUCTION AND USE THEM |
| abstract | An operon of hereby disclosed plasmides consists of a controllable Trp promotor or a cotrollable Tac promotor; a Shine-Dalgarno sequence effective as a ribosomal binding site; a translational start codon, wilst said Shine-Dalgarno sequence is separated from said start codon by from 6 to 12 nucleotides; a structural gene for single chain urinary plasminogen activator "scuPA" having 411 amino acids, wherein the triplet CGT is utilized as the codon for arginine; the triplet CTG is utilized as the codon for leucine; the triplet GTT is utilized as the codon for valine; the triplet CCG is utilized as the codon for proline; and the triplet GGT is utilized as the codon for glycine; and downstream of the structural gene at least one transcription terminator selected from the group containing trp A terminator and/or tet A/orf L-terminator from Tn 10. These plasmids are suitable for expressing a precursor protein of rscu-PA in Enterobacteriaceae, especially Escherichia coli. There is further described a process for producing novel plasmides comprising the step of: (i) removing the nic/bom-region and mutationally inactivating the tetracycline resistance gene of the plasmid pBR 322; (ii) inserting a multi-cloning site having the nucleotide sequence of FIG. 2 between the pBR 322 restriction sites Eco RI and Hind III; and (iii) inserting by means of the multi-cloning site a transcription terminator, a synthetic gene encoding scu-PA, and a Trp-promotor such that the gene is operatively linked to the promoter and the terminator. |
| priorityDate | 1989-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 127.