http://rdf.ncbi.nlm.nih.gov/pubchem/patent/SE-8605621-D0
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0fcf36dfec0fb3c2546b917cc56b8adf |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-41 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195 |
| filingDate | 1986-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1986-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | SE-8605621-D0 |
| titleOfInvention | HETEROMULTIMERIC PROTEINS AND THEIR MANUFACTURE |
| abstract | A method of in vivo production of a heteromultimeric protein, the subunits of which together but not separately are capable of generating a specific bioactivity comprising the steps: a) isolating at least two procaryotic genes coding for separate polypeptides; b) eliminating all ATG:s on the 5' end of each gene except the one coding for the starting methionine; c) cloning the genes resulting from step b) into at least one eucaryotic expression vector; d) introducing the expression vector(s) from step c) into a eucaryotic cell; and e) allowing said cell to express separately but simultaneously and coordinately the protein subunits coded for by the said genes resulting in generation of said specific bioactivity; a eucaryotic plasmid vector having introduced therein procaryotic DNA-constructs coding for the separate expression of the subunits of a heteromultimeric protein capable of generating a specific bioactivity in vivo; and the corresponding DNA-sequences and polypeptides. |
| priorityDate | 1986-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419537510 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID6137 |
Total number of triples: 18.