| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b3411ead77b0733882202a053e96db3d |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-703
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-705
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate |
1995-08-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ccbc55e2b6956fc7b3ac982c6f39dd7c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d674230c85c4caa91781ef79750bee25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4a43979835e0b0fcdfb0fd25fb6bff9 |
| publicationDate |
2003-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
SE-520315-C2 |
| titleOfInvention |
A method for determining the activity of a nucleotide polymerizing enzyme in a sample |
| abstract |
Method for determining the activity of a nucleotide-polymerising enzyme in a sample comprises: (a) capturing the enzyme with a monoclonal antibody (MAb) immobilised on a fixed carrier (the MAb binds the enzyme without significantly affecting its activity); (b) removing impurities and/or disturbance factors; (c) starting nucleotide polymerisation by adding a reaction soln. contg. a primer/template construction and nucleotide substrate; (d) providing reaction conditions to favour permanent association between the enzyme and primer/template prod. complex; (e) washing the nucleotide substrate, primer/template and reaction soln. to remove freshly synthesised polymer; (f) measuring the amt. of nucleotide incorporated with the polymer by standard techniques, and (g) determining enzyme activity. |
| priorityDate |
1995-08-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |