| titleOfInvention |
Vectors, plasmid, mammalian cell, human cell, system vector-host, method of toxic effect decrease, methods of selective marker insertion, methods of cells selection, nucleic acids, methods of expression detection, methods of polypeptide production, antibodies, methods of immune analysis, transgenic animals |
| abstract |
The invention provides viral and retroviral vectors that include a nucleic acid molecule encoding human cytosolic aldehyde dehydrogenase, or human glutamyl cysteine synthetase, or a combination thereof, and the invention also provides a mammalian isolated nucleic acid molecule encoding cytosolic aldehyde dehydrogen azinase. In addition, this invention provides a method of reducing the toxic effects of cyclophosphamide in a patient, comprising replacing a patient's blood-forming cells with blood-forming cells having a retroviral vector. Further, the present invention provides a method for introducing a selectable marker into a mammalian cell, which comprises transfecting the cell with a nucleic acid molecule encoding cytosolic aldehyde dehydrogenesis or human glutamylcysteine synthetase. Finally, the present invention provides a method for selecting mammalian cells expressing a target protein, which comprises: a) introducing into the cells a nucleic acid molecule, including a nucleic acid molecule encoding a target protein, and a nucleic acid molecule encoding a human cytosolic aldehyde dehydrogenase; b) culturing the resulting transfected cells; and c) selecting cells that express human cytosolic aldehyde dehydrogenase. |