| abstract |
FIELD: biotechnology. n SUBSTANCE: invention relates to a method of producing an antibody which binds to an antigen of interest. Method comprises producing a mammalian cell engineered for antibody expression, which is specific for the antigen of interest, insertion of one or more human gene segments V L and one or more human gene segments J L and insertion of one or more human V L gene segments and one or more human J L gene segments and an ectopic nucleotide sequence which codes an ADAM6 protein or a functional fragment thereof. n EFFECT: invention is effective for producing antibodies applicable for producing therapeutic agents for humans. n 19 cl, 9 dwg, 1 tbl, 4 ex |