http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2758950-C1

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68
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filingDate 2020-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4efc4e9d611ba0793031fdf90b754328
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_517bb1a9a5d676db4823b3a15ae497f9
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publicationDate 2021-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber RU-2758950-C1
titleOfInvention Method for diagnosing hepatic pathologies in dogs using cfa-mirnas
abstract FIELD: biotechnology.SUBSTANCE: invention relates to the field of veterinary science and biotechnology. Proposed is a method for using circulating caninefamiliaris (cfa) microRNAs (miRNAs) produced from hepatocytes as new non-invasive diagnostic biomarkers for accurately reflecting the type of ongoing hepatic pathologies in dogs with different morphologies of extrahepatic and intrahepatic congenital portosystemic shunts (ICPS). The method is implemented by extracting the total RNA from serum samples of dogs with the confirmed presence of ICPS and various hepatic pathologies, followed by reverse transcription with subsequent amplification in RT-PCR in real time using canine-specific primers for cfa-miR-122-5p, cfa-miR-34a-5p, cfa-miR-21-5p and cfa-miR-126-5p. All analyses were applied in three repeats for each sample, and the resulting average cycle threshold (Ct) for each tested microRNA was normalised relative to the average Ct of cfa-miRNA-16 (stably expressed endogenous control) and cel-miRNA-39 (exogenous spike in the control). The relative multiple change in each tested cfa-miRNA in the groups of ICPS compared to the corresponding healthy controls was then determined using the 2-ΔΔCtmethod. Tests have proved that cfa-miR-122 at a threshold value of ≥1.32 to 1.45 constitutes a diagnostic tool for microvesicular steatosis. Cfa-miR-34a at a threshold value of ≥ 1.15 to 1.23 constitutes a diagnostic tool for vacuolated hepatocytes with macrovesicular steatosis, as well as lipid or pigmented granuloma. Cfa-miR-21 at a threshold value of ≥ 1.07 to 1.23 constitutes a diagnostic tool for portal and parenchymal fibrosis. Cfa-miR-126 at a threshold value of ≥ 1.14 to 1.16 constitues a diagnostic tool for changing the hepatic blood supply in the form of arteriole proliferation and duct proliferation associated therewith in dogs with ICPS.EFFECT: invention provides a possibility of using the selected serum cfa-miRNAs as new non-invasive biomarkers for reflecting the histopathological and molecular events occurring in the liver in each type of shunt with high degree of sensitivity and specificity.1 cl, 1 tbl
priorityDate 2020-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA8F9A6
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID397778
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB7L0S9
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB2B0E2
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP41522
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCC0RJI0
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB2KEK1
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ8DFM1
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ7VRB6
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA3M3G1
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA2C4Y0
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB9JDU9
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ12ZS7
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA1UBR9
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ6CK57
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP0CO43
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID50018094
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID1495207
http://rdf.ncbi.nlm.nih.gov/pubchem/anatomy/ANATOMYID6239
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ8FK84
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ6CG57
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ5UXG8
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA7GXF6
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP0CO42
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB7L799
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ7VMY0
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ7NKT5
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ1RHP2
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID56655367
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ72I25
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCO61069
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ5NQ43
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID5327729
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCC0R000
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCC4KHI0
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA8MLG1
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB1MVZ4
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP59429
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCF6QRS1
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA9IVZ9
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA4SVI8
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID359834
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ2YRT7
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB2K6Z6
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB3R0X7
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ8RE31
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA1TQ96
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP81025
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCC4K7W8
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA6U880
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ8R7X4
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP69882
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB4T9I1
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ9PQP0
http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID12343
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ65UF7
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCC0R2Y9
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID57417918
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB9IZL5
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA8PR17
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID56941918
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCC1FMT0
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ9PKR0
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP67974
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID41279602
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA8PAA1
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ5HM20
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCC4KZM5
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA8EZJ5
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID61172971
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID60502039
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCO33007
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP67971
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB1YVE0
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID45136534
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP81423
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID10643737
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ0TKG7
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP67973
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA2BYR7
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP67969
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ5B2V8
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA7THY5
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ2P743
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID798009
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP67970
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ1C4P9
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID7040
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ8KD69
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ1IX93
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID282089
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA9IQ39
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ5NFR4
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCC0QB12
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID6495737
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ32J54
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA8F4T2
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ6NJ71
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP67968
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB2UYD1
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ72AR0
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID57975593
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA4SLY1
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB1LWQ5
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ0TMR7
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA4G7X8
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCC0Q812
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ1QZ94
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP16304
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ3B4C0
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA6XGL2
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ3KMP1
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP69442
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ1BU66
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB6J8H9
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA9H3J9
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID7814260
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB1Z772
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB8G1Y7
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID1245756
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB1LJN2
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA7GK41
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA2BTB8
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCB7NIF6
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA9FGE0
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP65201
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ7VFA6
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA3DJJ3
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA7GJ53
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ71WG7

Total number of triples: 1059.