http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2752805-C1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_90a9d8278c123a6fb5a1140dd68f7223 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-48 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 |
filingDate | 2020-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c6e3fba857858595965cf78179f4de2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb352e2e93e8287584930a9be1544203 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_adf4bd6e8dcb62d332ce5ff66f58e54e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41c68d361ab785a506d5e2ecac3240bc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9bd3f779b450068e56d86ba549a06c7d |
publicationDate | 2021-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2752805-C1 |
titleOfInvention | Method for measuring the concentration of polyglutamates of methotrexate by high-performance liquid chromatography with mass spectrometric detection (hplc-ms/ms) |
abstract | FIELD: medicine.SUBSTANCE: invention relates to a method for determining the concentration of methotrexate polyglutamates, including the collection of venous blood of a patient, separation of a sample of erythrocyte mass, isolation of methotrexate polyglutamates from it by means of a precipitation reaction and determination of the concentration of methotrexate polyglutamates using high-performance liquid chromatography with mass spectrometric detection (HPLC-MS/MS), moreover, the separated sample of the erythrocyte mass is frozen at a temperature of -80±2°C before the release of methotrexate polyglutamates from it, followed by thawing at a temperature of 6-10°C, while the precipitation reaction is carried out at a temperature of 6-10°C by introducing acidified acetonitrile into the sample, mixing it intensively, followed by centrifugation at 12000±10 rpm for 15±2 minutes and selecting the upper layer containing methotrexate polyglutamates for subsequent analysis using HPLC-MS/MS.EFFECT: wider range of methods for determining the concentration of methotrexate polyglutamates.8 cl, 1 dwg, 8 tbl, 4 ex |
priorityDate | 2020-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 523.