http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2744175-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8adaddc602ad7cb7f9e2d550681bb36d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y5-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1247 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2019-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_303a4e575d8c1599a0307d3564c3a549 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb14a6e9ac83278b25c92ded7030a9b4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8bff5cdffae8c046ef3f6bc979637735 |
| publicationDate | 2021-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2744175-C1 |
| titleOfInvention | High-performance single cell sequencing with reduced amplification error |
| abstract | FIELD: biotechnology. n SUBSTANCE: invention relates to the biotechnology. Described is a method of producing a sequencing library containing nucleic acids from multiple separate nuclei or cells, involving: obtaining a plurality of recovered nuclei or cells in primary sets of compartments, where each compartment contains a subpopulation of recovered nuclei or cells, and where nuclei or cells contain nucleic acid fragments; introducing a linear amplification mediator into cells or nuclei; amplification of nucleic acid fragments by linear amplification; treating each subpopulation of nuclei or cells to obtain indexed nuclei or cells, where the treatment involves adding to nucleic acid fragments present in isolated nuclei or cells a first compartment-specific index sequence for producing indexed nucleic acids present in isolated nuclei or cells, where the treatment involves ligating, primer lengthening, hybridisation, amplification or transposition; combining indexed nuclei or cells to produce combined indexed nuclei or cells, thereby obtaining a library for sequencing from multiple nuclei or cells. n EFFECT: invention extends range of sequencing methods. n 19 cl, 9 tbl, 2 ex, 23 dwg |
| priorityDate | 2018-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 209.