http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2730848-C2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_60b9c44857fe2d9d6716a0eabea0a78e |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-5031 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-27 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-62 |
filingDate | 2016-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_623c8d36f3fc2f6a5c41d2dea0a688c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5bc943b522809214ab7aa02989757e04 |
publicationDate | 2020-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2730848-C2 |
titleOfInvention | Methods for amino group pegylation to obtain site-specific conjugates of proteins |
abstract | FIELD: medicine.SUBSTANCE: group of inventions relates to a method of producing a protein-polyethylene glycol conjugate. Method comprises preparing an aqueous protein solution containing protein, a buffer solution which provides the desired pH, and a chelating agent, wherein said protein is insulin, said buffer solution, providing the desired pH, is an acetate or citrate, and said chelating agent is selected from a group consisting of aminopolycarboxylic acid, hydroxyaminocarboxylic acid, N-substituted glycine, 2-(2-amino-2-oxoethyl)aminoethanesulfonic acid (BES) and deferoxamine (DEF); adding to the aqueous protein solution a boron-containing reducing agent and methoxypolyethylene glycol aldehyde, wherein the boron-containing reducing agent and methoxypolyethylene glycol aldehyde are taken in molar ratio from 25:1 to 1.5:1; and reacting methoxy polyethylene glycol aldehyde and protein to form a protein-polyethylene glycol conjugate, wherein the buffer solution during reaction provides pH of the aqueous protein solution from 3.88 to 4.27, and as a result of said interaction of methoxy polyethylene glycol aldehyde with said protein conjugate of PEG and protein, pegylated in one position is formed. Group of inventions also relates to a method of producing an insulin-polyethylene glycol conjugate and a method of producing delayed release microspheres containing a protein conjugate with polyethylene glycol.EFFECT: group of inventions enables to obtain pegylated proteins with high output, as well as higher clinical effectiveness in diabetes and other diseases.35 cl, 16 dwg, 19 ex |
priorityDate | 2015-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 319.