http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2729410-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_91371b4cdcc5125f3842f8ac06a70397 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-84 |
| filingDate | 2020-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f51271aebc0676d66fae0fb27beeb573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e29973ddce9160531909414a54a279c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba69fdc08570a309a72bdc9526b2b8a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a6812fb532d88f5b66cf24f2c8447d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e241374a6a418dad39e2bcf6554f802 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b21e88bac7fac2f33add6b9efdbd9819 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a456c4d7fe32cd7bd9652f1ae5d9fcc3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03a620c9e27d57f18dd19b3f39a0b373 |
| publicationDate | 2020-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2729410-C1 |
| titleOfInvention | Industrial method for microbiological synthesis of penicillin g acylase escherichia coli enzyme |
| abstract | FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and a method for industrial cultivation of a recombinant strain E.coli BL21 (DE3)/pMD704 – a producer of penicillin G acylase. Method provides production of ECOPGA preparation in industrially significant amounts. Method provides growing of inoculum based on E.coli BL21 (DE3)/pMD704 strain to middle of logarithmic phase of growth on nutrient medium using glucose as carbon source at temperature of 38 °C, stirring at 200 rpm for 3–5 hours, seed transfer fermenter with inoculum, further cultivation of strain in fermenter at 22–23 °C on medium containing sources of nitrogen, carbon, inorganic salts, with concentration of dissolved oxygen of 80–90 % at maximum mixing rate of 1,200 rpm, maximum level of aeration 1 volume of air/min : 1 volume of culture fluid. After cultivation, the produced biomass is precipitated and destroyed. End product (ECOPGA) is extracted in the form of an enzyme paste by precipitation with the help of polyethylene glycol.EFFECT: invention increases output of biomass and functional-active penicillin G acylase from unit volume of culture medium; as a result, a preparation ECOPGA is obtained, which is suitable for producing biocatalysts for synthesis of cephalosporin acids.1 cl, 3 dwg, 1 tbl, 4 ex |
| priorityDate | 2020-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 98.