http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2723160-C1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_99cd2888b2026702c522c60b1544ff90 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-658 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 |
filingDate | 2019-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02098d699b7dd9963ec2a5259749d9a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd2f50247a4c9165aa22d5a5f89c1a66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9558bd9597eb9cff04a1ae412cb1040c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_963a084166ffe8e8c0482a394532dc00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1cefeeb9a260c3e11540a85af06217a0 |
publicationDate | 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2723160-C1 |
titleOfInvention | Method of detecting and determining dna with a given sequence using giant raman scattering spectroscopy |
abstract | FIELD: biotechnology.SUBSTANCE: method of DNA determination in biological fluids, which consists in preparation of nanoparticles of noble metals acting as a GRS-amplifier; target hybridisation with a targeted covalently GRS-labeled complementary oligonucleotide; applying a sample with the analyzed compound to the surface of liquid sample nanoparticles with the analyzed compound to form a structure directed structurally by non-covalent interactions, including a DNA to be determined, a GRS mark and an oligonucleotide; the obtained hybridised GRS-labeled duplex is detected by giant Raman scattering spectroscopy. Qualitative and quantitative DNA content is determined from the position and intensity of the bands on the recorded spectra. Method is applicable for detecting small amounts of DNA against the backdrop of a large amount of foreign components of the analyzed biological fluids.EFFECT: high sensitivity, selectivity and rapidness.14 cl, 22 dwg, 2 tbl |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2764807-C1 |
priorityDate | 2019-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 71.