http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2721123-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2074383949c79d189ca7f9df35a08ed5 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 |
| filingDate | 2019-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b0dc524ad6a9af14a9cd71f2edfb5bb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f32a9f6962924437711ec2f907646a9d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b08a35b86fac6ed8925ca2b061c39584 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0231b0bfcdaab26e0c2b8aa4d1b87022 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e35b7fe9c085958a0f2157159463de2b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a51a9aedf6f5eeb95cadcc7bbba6bc93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e86bdd6191fa8a8bb561278ac6d2b06 |
| publicationDate | 2020-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2721123-C1 |
| titleOfInvention | Recombinant pseudo-adenoviral particle strain expressing the chlamydia trachomatis chimeric gene mbl-ct666, a method for preparing it, an immunogenic composition for protection against human urogenital chlamydiosis |
| abstract | FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and concerns a strain of recombinant pseudo adenoviral particle expressing a chimeric gene MBL-CT666after its introduction into an individual's body. A recombinant pseudo adenoviral particle strain expressing the chimeric gene MBL-CT666is obtained, which contains the nucleotide sequence SEQ ID NO: 1 chimeric gene MBL-CT666, which after introduction into an individual's body produces a chimeric protein MBL-CT666in the form of the amino acid sequence SEQ ID NO: 2, inducing protection against human urogenital chlamydiosis. Method of obtaining a strain of recombinant pseudo adenoviral particle expressing chimeric gene MBL-CT666consists in that strain is obtained by homologous recombination in E. coli strain BJ5183 between plasmid pShuttle-CMV (#240007, pShuttle-CMV vector, Agilent, US), carrying cassette with chimeric gene MBL-CT666cloned from plasmid pAL2-T, and adenovirus DNA of human serotype 5 with deletion in region E1 and E3 of adenovirus genome, wherein assembly of recombinant pseudo adenoviral nanoparticles is carried out in cells of line HEK293, Human embryonic kidney 293, after transfection of recombinant DNA pAd-MBL-CT666, wherein as a result of construction, the expression cassette comprises a human cytomegalovirus CMV promoter, target gene – chimeric gene MBL-CT666, pA signal of polyadenylation and is in the region of E1 deletion of adenovirus genome, wherein polymerase chain reaction with specific primers is used, obtaining an immunogenic composition containing a recombinant pseudo adenoviral particle strain expressing the chimeric gene MBL-CT666for use in an amount of not less than 2×10PFU/ml, wherein it contains a pharmaceutically acceptable buffer solution of up to 0.5–1.0 ml, a dose volume of 0.5–1.0 ml in a flask.EFFECT: invention enables to apply an immunogenic composition containing a strain of a recombinant pseudo adenoviral particle expressing a chimeric gene MBL-CT666as a prime component of a chlamydial vaccine for protection against human urogenital chlamydiosis.4 cl, 11 dwg, 2 tbl, 7 ex |
| priorityDate | 2019-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 75.