http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2708917-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_99cd2888b2026702c522c60b1544ff90 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-9406 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-52 |
| filingDate | 2017-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e753bc09bd9e62a70fd59acf18266a6e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_963a084166ffe8e8c0482a394532dc00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a13d46a82128c032672f39483629890e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1cefeeb9a260c3e11540a85af06217a0 |
| publicationDate | 2019-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2708917-C2 |
| titleOfInvention | Test system for determining low-molecular markers of neurotransmitter metabolism in samples of biological fluids, method for production thereof and method for determining catecholamines and metabolites using thereof |
| abstract | FIELD: medicine; biotechnology.SUBSTANCE: group of inventions relates to biotechnology and can be used in medical diagnostics and biochemical studies for quantitative detection of catecholamines and their metabolites in biological fluids. Disclosed are a test system and a method for rapid determination of low-molecular markers of neurotransmitter metabolism in biological fluid samples, as well as a method of producing a test system. Test system is a plate with chitosan film coated on the surface of the wells with immobilized peroxidase of plant origin of 0.5 to 1.5 mcm. Peroxidase is contained in amount of 2 to 16 pmol per 1 mmarea of film, and chitosan - from 15 to 60 mg per 1 mmarea of the film. Chitosan has molecular weight distribution of 50–220 kDa and degree of deacetylation of not less than 75 %. Method for rapid determination of catecholamines and metabolites thereof involves applying a sample of biological fluid on a well of a test system with subsequent introduction of hydrogen peroxide and a derivatizing agent with formation of fluorescent derivatives, excitation of fluorescence in the sample with recording of obtained signals.EFFECT: invention enables to determine simultaneously several markers of neurotransmitter metabolism selected from noradrenaline, dopamine, epinephrine and their metabolites, selected from homovaniline and vanililmedal acids, in a large number of biological fluid samples with achievable reproducibility of the results of not less than 99 % and analysis time of up to 12,5 s per one sample.15 cl, 6 dwg, 4 tbl, 13 ex |
| priorityDate | 2017-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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