http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2708556-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_68235a5e3014d64e15d3c31ce7860832 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
| filingDate | 2018-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e730c6a039acdd442e110d4829c2e88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b25a2249b19595d0fa93c9d352416b64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ff077ff4cd728093eab28c938b39a7b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1caec0b95cb7c30590212e0f1dda62c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_76261d43e17105b37f1aeef11f60b93a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4a7dace2dc01da95da2ae595dc857ce |
| publicationDate | 2019-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2708556-C1 |
| titleOfInvention | RECOMBINANT PLASMID DNA p280_2GM CODING POLYPEPTIDE WITH PROPERTIES OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR OF HUMAN, STRAIN ESCHERICHIA COLI SG 20050/p280_2GM - PRODUCER OF POLYPEPTIDE WITH PROPERTIES OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR OF HUMAN AND METHOD OF OBTAINING OF SAID POLYPEPTIDE |
| abstract | FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, particularly to genetic engineering, and is an in vitro constructed recombinant plasmid DNA containing two copies of a human granulocyte-macrophage colony-stimulating factor (GM-CSF) synthetic gene, Escherichia coli strain SG20050/p280_2GM is a producer of said polypeptide and a method of producing a polypeptide with GM-CSF properties. Recombinant plasmid DNA p280_2GM codes a polypeptide with properties of granulocyte-macrophage colony-stimulating factor (GM-CSF) of a person characterized by the following features: codes the amino acid sequence of the mature human GM-CSF; has molecular weight of 2.95 MDa (4,422 base pairs); consists of: PsTI/BamHI(pol)-fragment of plasmid DNA p280GM (1,484 base pairs) containing C-terminal part of beta-lactamase gene, tandem of promoters of tryptophan operon E. coli and synthetic gene GM-CSF of human, as well as EcoRI(pol)/PstI (2,938 base pairs) – a fragment of the same plasmid containing the synthetic human GM-CSF gene and the N-terminal portion of the beta-lactamase gene; contains: – tandem promoters of tryptophan operon E. coli; two copies of human synthetic GM-CSF gene; transcription terminator t0 of lambda phage; as a genetic marker, a beta-lactamase gene determining the resistance of E. coli transformed plasmid p280_2GM to ampicillin antibiotics; unique recognition sites by restriction endonucleases located at the following distances to the right from EcoRI site (279 base pairs) – BamHI – 912 nucleotides; PstI – 3,396 nucleotides.EFFECT: invention increases output of polypeptide with properties of granulocyte-macrophage colony-stimulating factor.3 cl, 8 dwg, 4 tbl, 15 ex |
| priorityDate | 2018-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.