http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2708337-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c631983469a831921ef058e527cf30a9 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-155 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-204 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2522-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-191 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-161 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 |
| filingDate | 2015-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_272ba348da7d2816cf4ee5981e81fe15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1cf6f3ba5fd82a499ea713daed64eaa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9cd22e73eef5d31657cc14026bf9370a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5673a26977fb07c6fc01f81b398b6e98 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0f65c33f69b84d52b28bb7ddde17339 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44b1e363ecb872906688ed9af85a7afd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31ed6fc12100a4a56b43a456424b5c7d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5f2b37127ab5ea64b154c8bdf1dbe39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b676f4cdf60e2ddbb1be2e434380a4e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c5f3656140df69c7f2fd65839d9dd3da |
| publicationDate | 2019-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2708337-C2 |
| titleOfInvention | Methods and compositions for dna profiling |
| abstract | FIELD: biotechnologies.SUBSTANCE: invention relates to the biotechnology. Described is a plurality of primers for use in constructing a human DNA profile, comprising two sets of primers. First set of primers contains primers which specifically hybridise with the first target sequence and in combination with DNA polymerase and nucleotides amplify a short amplification product, having a short amplification product length, wherein the short amplification product sequence comprises single nucleotide polymorphism (SNP), where the first set of primers contains one or more primers, each comprising a nucleic acid sequence presented by any of SEQ ID NO: 111–402. Second set of primers contains primers which specifically hybridise with the second target sequence and in combination with the DNA polymerase and nucleotides amplify a long amplification product having a long amplification product length, where the length of the short amplification product is shorter than the length of the long amplification product, where the sequence of the long amplification product contains a tandem repeat, where the second set of primers contains one or more primers, each comprising a nucleic acid sequence presented by any of SEQ ID NO: 1–110. At least one of the primers in the plurality of primers has a melting point which is less than 60 °C, or has a length of at least 24 nucleotides or contains a homopolymeric nucleotide sequence or a combination thereof, where at least one of the primers in the plurality of primers has a melting point which is less than 60 °C, and has a length of at least 24 nucleotides and where a plurality of primers comprises a first set of primers comprising up to 20,000 primers, and a second set of primers comprising up to 1200 primers. Described is a method of constructing a DNA profile, involving: providing a nucleic acid sample, amplifying a nucleic acid sample with a plurality of primers which are specifically hybridised with at least one target sequence comprising mononucleotide polymorphism (SNP), and with at least one target sequence containing a tandem repeat, in a multiplex reaction to obtain amplification products, where at least one of the primers in the plurality of primers has a melting point which is less than 60 °C, or has a length of at least 24 nucleotides, or contains a homopolymeric nucleotide sequence or a combination thereof, where at least one of the primers in the plurality of primers has a melting point which is less than 60 °C and has a length of at least 24 nucleotides, and determining genotyping amplification of at least one SNP and at least one tandem repeat, thereby obtaining a DNA profile of the nucleic acid sample. In addition, a method of constructing a library of nucleic acids, comprising: providing a nucleic acid sample, and amplifying a nucleic acid sample with a plurality of primers which are specifically hybridised with at least one target sequence comprising mononucleotide polymorphism (SNP), and with at least one target sequence containing a tandem repeat sequence, in a multiplex reaction to produce amplification products, where at least one of the primers in the plurality of primers has a melting point which is less than 60 °C, or has a length of at least 24 nucleotides, or contains a homopolymer nucleotide sequence or a combination thereof, wherein at least one of the primers in the plurality of primers has a melting point which is less than 60 °C and has a length of at least 24 nucleotides.EFFECT: invention enables to obtain a human DNA profile.32 cl, 25 dwg, 5 tbl, 7 ex |
| priorityDate | 2014-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 405.