abstract |
FIELD: biotechnology. n SUBSTANCE: invention refers to biotechnology and molecular biology. Disclosed is an in vitro method of assembling nucleic acids using a nuclease agent - a complex of gRNA-Cas, nuclease with zinc fingers or effector nuclease. n EFFECT: invention can be used in applied biotechnology and medicine due to increased efficiency of producing long nucleic acids. n 43 cl, 19 dwg, 1 tbl, 14 ex |