| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50eb752940fa4aa36756a6bc9f6f3504 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-0387
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2015-8527
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2207-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-072
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0278 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-7051
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0278
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 |
| filingDate |
2015-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2019-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6f3ca937e60fa10b86d28df91ef5c0c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7727bb04135a90f2bf6ff86c3f42642d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_052c655dd3bbe74281decd92a1900b45
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8dfd4fd1f80b9a06d842af2667a6c03
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e09464a433b89ea9bf5043193b34ce0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67490e655088eb269019707ffe97f943 |
| publicationDate |
2019-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
RU-2704283-C2 |
| titleOfInvention |
Methods and compositions for modifying target locus |
| abstract |
FIELD: biotechnology. n SUBSTANCE: invention relates to biotechnology and molecular biology. Disclosed are methods and compositions for modifying one or more target loci in a cell. Such methods include providing a cell containing a first polynucleotide coding a first selective marker operably linked to a first promoter active in a cell, wherein the first polynucleotide further comprises a first recognition site for the first nuclease agent. First nuclease agent is introduced into the cell, wherein the first nuclease agent induces a single- or double-strand break at the first recognition site. First targeting vector containing a first polynucleotide insert flanked by the first and second homologous arms, which correspond to the first and second target sites located close enough to the first recognition site, is additionally introduced into the cell. That is followed by identifying at least one cell containing in its genome the first polynucleotide insert integrated into the target locus. n EFFECT: invention can be applied in medicine. n 36 cl, 3 dwg, 2 tbl |
| priorityDate |
2014-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |