http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2702766-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_766802ff2e94615e2c615daf957853d5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-978 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-03007 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 |
| filingDate | 2015-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e7dd1c71f03297b18130609f20d51cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f99981f5c69617324fa02a40497e4e0e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa95a59e669b7ca2b1f83e6e6767046a |
| publicationDate | 2019-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2702766-C2 |
| titleOfInvention | Ureohydrolases as dominant selective markers in yeast |
| abstract | FIELD: chemistry.SUBSTANCE: invention relates to molecular biology. Disclosed is a method of culturing a microorganism selected from genera Saccharomyces sensu stricto, Kazachstania, Naumovozyma, Nakaseomyces and Vanderwaltozyma, in the presence of guanidinobutyrate as the only nitrogen source, including the incorporation into the said microorganism of a nucleic acid molecule containing a nucleotide sequence coding guanidinobutyrase with number EC 3.5.3.7 and operably linked to promoter and terminator sequences, culturing said microorganism such that a nucleic acid molecule encoding guanidinobuturase is expressed in said microorganism, and culturing said microorganism in the presence of guanidinobutyrate as a single nitrogen source. Also disclosed is a microorganism for growth on guanidinobutyrate as a single source of nitrogen, selected from genera Saccharomyces sensu stricto, Kazachstania, Naumovozyma, Nakaseomyces and Vanderwaltozyma containing nucleotide sequence coding guanidinobutirase with EC number 3.5.3.7. Also disclosed is a method for changing the genome of a microorganism specified in genera Saccharomyces sensu stricto, Kazachstania, Naumovozyma, Nakaseomyces and Vanderwaltozyma.EFFECT: invention provides efficient use of guanidinobutyrase as a selective marker.10 cl, 10 dwg, 5 tbl, 1 ex |
| priorityDate | 2014-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1535.