http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2701648-C2

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grantDate 2019-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2019-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber RU-2701648-C2
titleOfInvention Method for carrying out real time pcr for genotyping cattle on alleles a and b of the csn3 gene
abstract FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology and genetics of livestock. Method is proposed for carrying out real time PCR for genotyping cattle for alleles A and B of the CSN3 gene, in which used 5'-fluorescently-labeled direct A-allele-specific primer CSN3-A: 5'-FAM-ACTGTAGCTACTCTAGACGA-3', 5'fluorescently-labeled direct B-allele-specific primer CSN3-B: 5'-VIC-ACTGTAGCTACTCTAGATGC-3', reverse common primer CSN3-R: 5'-GCTCTCAATAACTTCTGGAGAA-3', anti-primer CSN3-S, labeled with a fluorescence quencher with 3'end of oligonucleotide: 5'-TCTAGAGTAGCTACAGT-3'-BHQ1.EFFECT: invention makes it possible to efficiently carry out the genotyping of cattle by the CSN3 gene based on real time PCR in the format of hybridization-fluorescence detection.1 cl, 2 dwg, 1 tbl
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