| abstract |
FIELD: biotechnology. n SUBSTANCE: invention relates to biotechnology, genetic and protein engineering. Method for extraction and purification of recombinant protein, analogue of human kappa-casein fragment, involves cultivation of HEK293T-producing cells expressing recombinant EL1 protein, concentration and purification of the culture medium first using cation-exchange chromatography on SP-sepharose, and then using nickel-affinity chromatography on IMAC-sepharose, successive elution from impurity proteins and target product sorbents using a system of eight buffer solutions and subsequent purification of the end product by dialysis against 150 mM NaCl. n EFFECT: invention increases specific activity and purity of the end product. n 1 cl, 7 dwg, 4 ex |