http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2685472-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7eba1b776070a719d8390e6e6db1b0bc |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
| filingDate | 2017-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf6f1054ece4f6ccaa743ec74b7e554e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88ff07f231d83adeb28f1dd1687ccbb8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0294149a31c990b06b6e210008bae37e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df153edeeb483ce66a993bf9bb568661 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_097e47898a47022e7fe93587885cadfe |
| publicationDate | 2019-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2685472-C2 |
| titleOfInvention | The method of preparation of autologous human monocytes for the treatment of burn wounds |
| abstract | The invention relates to the field of biotechnology, in particular to the preparation of autologous human monocytes for the treatment of burn wounds. The method involves isolating venous blood monocytes by gradient centrifuging (on a ficoll gradient and a double percoll gradient). Then, stimulation with 100 μM of non-hydrolyzable adenosine analog 5'-N-ethylcarboxamido adenoses is carried out in the presence of 20 ng / ml IL-4 and 20 ng / ml GM-CSF, cultivation for 72 hours in a CO 2 incubator in a humid atmosphere of 5% CO 2 with + 37 ° C, then frozen in serum-free medium Bambanker, followed by storage for 24 hours at -80 ° C and then in liquid nitrogen at -196 ° C. The invention allows to expand the arsenal of funds. 5 il., 1 pr. |
| priorityDate | 2017-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 75.