| abstract |
The invention relates to veterinary medicine, and in particular to a method of manufacturing erythrocyte diagnosticum for the diagnosis of pullorosis-typhoid birds. The method includes obtaining the bacterial mass of Salmonella gallinarum pullorum, isolating the antigenic fraction from it, Treating the bacterial mass with a surfactant with adding soda or alkali in distilled water at 93-96 ° C with further sensitization of formalized erythrocytes, their purification and obtaining the desired product as 10% suspension. As a surfactant, a 0.8-1.2% aqueous solution of Desmol is used, taken in a final weight concentration of 0.1-0.5%. The sensitization of formalinized erythrocytes is carried out in the presence of sodium salt of chitosan succinate, taken in a final weight concentration of 0.5-1.5%. EFFECT: obtaining erythrocyte diagnosticum for diagnosing pullorosis-typhoid in hypersensitive birds. 3 pr. |