http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2677336-C2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_08e00d6c0f225b2092510b9d4c97635d |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-025 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-025 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
filingDate | 2015-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d9e24952058d64db3bc1475094c10ff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c3f0642aa422e68311af38e6180337e4 |
publicationDate | 2019-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2677336-C2 |
titleOfInvention | Method for obtaining virus-like particles of human papillomavirus |
abstract | FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Method for producing virus-like particles (VLPs) based on the human papillomavirus L1 protein (HPV) is described, including: (a) cultivating transformed yeast expressing HPV L1 protein; (b) performing ammonium sulfate precipitation in the product of transformed yeast cultured in step (a) to obtain a precipitate; (c) treatment of the precipitate obtained by precipitation with ammonium sulfate, NaCl, KCl or NaCOat a concentration in the range from 0.35 to 0.60 M, followed by incubation and removal of the resulting insoluble proteins; and (d) performing chromatography on the product obtained in step (c) to obtain virus-like particles (VLPs) based on the human papillomavirus L1 protein (HPV).EFFECT: invention expands the arsenal of methods for producing virus-like particles based on human papillomavirus L1 protein.8 cl, 19 dwg, 4 ex, 3 tbl |
priorityDate | 2014-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 128.