| abstract |
FIELD: medicine.SUBSTANCE: invention relates to medicine, in particular to experimental pharmacology, and can be used to inhibit the nuclear factor kappa B (NF-kB). This method involves the addition of bacterial lipopolysaccharide at a concentration of 1 mcg/ml to the mononuclear blood cells of Wistar rats freshly isolated by the standard method of velocity sedimentation on gradient of Ficoll, which is followed by adding 5-hydroxynicotinate 3-(2,2,2-trimethylhydrazinium) potassium propionate, dissolved in phosphate-buffered saline, at a final concentration of 35 mcg/ml.EFFECT: method allows to evaluate the inhibitory NF-kB activity of 5-hydroxynicotinate 3-(2,2,2-trimethylhydrazinium) potassium propionate.1 cl, 1 tbl, 1 ex |