http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2672378-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e5421b664c7c22ec24417a9e81faa650 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 |
| filingDate | 2017-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a8cb39fe2319a203dd26a027d8875b9d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e34a1fa8caa29fba5fed1d4ad2761a9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd5af7ebac8735aff3060a503ad0146c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e8e391ab2a8e9191909068ceb4346b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e8326fab924c4062b5b955da8e0776d |
| publicationDate | 2018-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2672378-C1 |
| titleOfInvention | Method for dna isolation from plants, suitable for pcr |
| abstract | FIELD: microbiology. n SUBSTANCE: invention relates to molecular biology, in particular to a method for isolating DNA from plants, suitable for PCR. Sample under study is homogenized in a non-extracting buffer containing TE buffer and polyvinylpyrrolidone (PVP). Next, perform primary lysis using TRITON X-10. Resuspend the pellet in TE buffer with PVP, followed by centrifugation. Remove the supernatant. Stage is repeated 2–3 times. Then a lyse solution containing GuSCN, DTT, EDTA, TRIS-HCI is added to the precipitate, mixed and proteinase K and/or RNase A are added. Next, chloroform is extracted and the DNA is concentrated with isopropanol. After DNA precipitation, the precipitate is washed twice, first with 70 % ethanol, and then with acetone. Dry and elute the DNA in TE buffer. Purity of the isolated DNA free of PCR inhibitors relative to A260/A280 protein is 2.0 ± 0.09. n EFFECT: invention allows to obtain a DNA solution of high quality and a high concentration of predominantly nuclear genome, suitable for laboratory research, including PCR. n 1 cl, 4 dwg, 2 ex |
| priorityDate | 2017-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.