http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2663118-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e5421b664c7c22ec24417a9e81faa650 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-098 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2523-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2513-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P3-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0678 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0676 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
| filingDate | 2017-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c82e556d76c9f9660b18ac60a89c1633 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bce990d4020de2f11c70be51175a822d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8fb8b355c9af479d32e11f87b5bbdd2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce67990591a8e6919bde3a14341ceab3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74edf48dd0bd3f4a86f8f09e8c860ea0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b3104436e0c5d7b15187b85c484dd7e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e94b3711b9bc1c4c7abb4c34d9cc07e2 |
| publicationDate | 2018-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2663118-C1 |
| titleOfInvention | Cell product of insulin-producing mammalian cells and use thereof for diabetes mellitus therapy |
| abstract | FIELD: biotechnology. n SUBSTANCE: invention relates to biotechnology. Invention is a method for producing a cellular product of insulin-producing human cells, comprising producing epithelial progenitor cells from an epithelial tissue biopsy material and their subsequent pancreatic differentiation into cells capable of glucose-dependent secretion of insulin, in which pancreatic cells are differentiated in two stages in a culture medium for eukaryotic cells providing a calcium ion concentration in the range of 0.5 mM to 2.5 mM: at the first stage, cells are cultured for 4–15 days in a culture medium containing at least blood serum – 2–20 vol. %, glutamine – not less than 1 mM, epidermal growth factor – 1–300 ng/ml, transferrin – not less than 0.1 mcg/ml, sodium selenite – 0.1–20 ng/ml, retinoic acid – 0.1–20 mcM, isoproterenol – 0.1–10 mcM; at the second stage, cells are cultured for 4–15 days in a culture medium containing at least blood serum – 2–20 vol. %, glutamine – not less than 1 mM, epidermal growth factor – 1–300 ng/ml, transferrin – not less than 0.1 mcg/ml, sodium selenite – 0.1–20 ng/ml, retinoic acid – 10 nM – 20 mcM, nicotinamide – 1–100 mM, hepatocyte growth factor – 1–300 ng/ml, dexamethasone – 0.01–5 mcM, where the cultivation in both stages is carried out at 37 °C in the presence of 5 % CO 2 . Group of inventions includes a cellular product of insulin-producing mammalian cells, as well as a method of diabetes mellitus replacement therapy using a cell product. n EFFECT: invention provides a simplified technology of producing insulin-producing cells, allows producing not less than 70 % of the functionally active insulin-producing cells in the cell culture that has undergone differentiation. n 14 cl, 10 tbl, 7 ex, 11 dwg |
| priorityDate | 2017-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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