http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2650871-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_95a2f6cf8f7faa2e9c94695d49f23ee9 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2017-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15fb91982084e1e1bc9b3db5c3d7d910 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbcb555084425621fcbf3fcacfae25be http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_560f6425a22a347784e804e5cd59c4c8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8bcbaa500e7a72816a3016fd1a345b32 |
| publicationDate | 2018-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2650871-C1 |
| titleOfInvention | Recombinant plasmid providing synthesis of barnase in cells of escherichia coli, escherichia coli strain - producer of barnase and method for producing barnase |
| abstract | FIELD: biotechnology. n SUBSTANCE: group of inventions refers to biotechnology and concerns production of recombinant barnase. Recombinant plasmid pET-STII-Ba is proposed that provides synthesis in Escherichia coli cells of barnase having the amino acid sequence of SEQ ID No. 3. This plasmid was obtained by cloning a recombinant DNA fragment with nucleotide sequence of SEQ ID No. 1 to the plasmid vector pET39b at the NdeI/HindIII restriction sites. Recombinant strain of Escherichia coli BL21(DE3)/pET-STII-Ba, containing recombinant plasmid pET-STII-Ba and producing barnase. Method for producing barnase with amino acid sequence of SEQ ID No. 3, from biomass strain Escherichia coli BL21(DE3)/pET-STII-Ba includes the isolation of protein from the periplasm of cells grown at a reduced temperature of 25 ºC, and subsequent purification by chromatography on phosphocellulose and a cation exchanger. n EFFECT: group of inventions allows to increase and stabilize the production of recombinant barnase upon accumulation of the latter in the periplasm of cells grown at a reduced temperature 25 °C. n 3 cl, 4 dwg, 6 ex |
| priorityDate | 2017-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 77.