| abstract |
FIELD: biotechnology. n SUBSTANCE: single-chain antibody for treatment or diagnosis comprising a single polypeptide containing the following domains located relative to each other from the N-terminus to the C-terminus is provided as follows: VL 1 -CL 1 -CLH linker1-VH 1 -CH1 1 -sharnir1-CH2 1 -CH3 1 -HD linker-VL 2 -CL 2 -CLH linker2-VH 2 -CH1 2 -sharnir2-CH2 2 -CH3 2 , where each of the CLH linker1, CLH linker2 and HD linker contains an amino acid sequence cleaved by the endopeptidase at the N- and C-termini. Also, a polynucleotide encoding this single-chain antibody, a vector comprising such a polynucleotide, a host cell comprising this vector, a method for production of this single-chain antibody by the said host cell are presented. n EFFECT: invention extends the arsenal for antibodies production. n 112 cl, 6 dwg, 3 ex |