| abstract |
FIELD: medicine. n SUBSTANCE: CD4+- bone marrow cells are cultured in CO 2 -incubator at 37°C, 5% CO 2 and 100% air humidity for 24 hours. Incubation is carried out in the culture medium of the following composition: 90% of RPMI-1640 medium, 10% of ETS, 280 mg/l of L-glutamine, 50 mg/l of gentamicin, 50 mcM of phosphatidylinositol-3-kinase inhibitor (PI3K). n EFFECT: increased production of erythropoietin. n 1 tbl, 1 ex |