| abstract |
FIELD: biotechnology. n SUBSTANCE: A Pseudomonas (PE) ectotoxin is proposed with substitution of one or more amino acid residues at L423, F443 and L477 positions. Also a chimeric molecule containing a targeting agent fused to PE and a molecule containing a targeting agent conjugated to PE. In addition, nucleic acids encoding PE and chimeric molecule; a recombinant expression vector; a host cell for PE production; a pharmaceutical composition for cell growth inhibition; a method for cancer treatment or prevention in a mammal; a method for target cell growth inhibition; methods for production of PE, chimeric molecule containing a targeting agent fused to PE, and molecules where PE is covalently bound to the targeting agent are described. n EFFECT: A Pseudomonas exotoxin of this invention exhibits reduced immunogenicity while maintaining cytotoxicity. n 23 cl, 12 dwg, 20 tbl, 16 ex |