http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2619643-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_07963b962430f84338c6296200c8886b |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5041 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2015-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a2b93d8bfebe71b399ab8dcff2d0237 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d080a6b5bcb19440cff13cec0d5abdf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_845564d3accb0086ed03bde470e71436 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e0b527da3b91606b361b71e5beee2c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_227e65ca32ab38115960b25c106e2240 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d76bfc91688b25a49eecc1a4a8852b6b |
| publicationDate | 2017-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2619643-C1 |
| titleOfInvention | Pigas cell line containing stat-1 transcription factor binding site stable-integrated in genome |
| abstract | FIELD: biotechnology. n SUBSTANCE: invention relates to cellular engineering, and can be used to determine the biological activity of IFN-γ. A human melanoma cell line PiGAS with the registration number VKPM H-161 is obtained by transfection of MelP parent human melanoma cell line with pGAS(x6)-Luc//Neo plasmid encoding 6xGAS-Luc reporter construct consisting of sequence of SEQ ID NO: 1, comprising 6 repetitions of the STAT-1 transcription factor binding site, minimal SV-40 promoter, and Photinus pyralis (Luc) gene, as well as sequence SEQ ID NO: 2, comprising the Neo resistance gene to the selective antibiotic G418 under the control of its constitutive PGK-1 promoter. n EFFECT: obtaining of a reporter cell line that allows determination of bioactive IFN-γ by of STAT-1 - signaling pathway activation detection. n 6 dwg, 5 ex |
| priorityDate | 2015-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 125.