| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_146c193e5ddbf78ec4e28994d4b83805 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-435 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate |
2015-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2017-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_172fcaf1c6ac9d7b33dbef28b9fffc44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80ae4d0ece8e7fe5c338d4ab7d39287c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f59132be4b526e5ec7f0ed924dd9ce48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3073bab4c14d4e076eed447abdd781d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8511a6ebf0f5bf4050cfa38d17dee999
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ddb8f1097e2b1d240d7c7c2fb2c8a0b |
| publicationDate |
2017-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
RU-2619170-C2 |
| titleOfInvention |
RECOMBINANT PLASMID DNA pER-ARNS3, ENCODING FUSION PROTEIN CAPABLE OF AUTOCATALYTIC SPLITTING WITH FORMATION OF ARNS3, ESCHERICHIA COLI STRAIN C3030/pER-ARNS3 PRODUCER OF SAID PROTEINS AND METHODS FOR RECOMBINANT ARNS3 PRODUCTION |
| abstract |
FIELD: chemistry. n SUBSTANCE: invention refers to biochemistry, namely to recombinant plasmid DNA pER-ARNS3 providing the synthesis of a hybrid polypeptide comprising ARNS3 and mini-intein Ssp DnaB, in Escherichia coli cells. The said plasmid DNA comprises BsaBI/Eco0109I-DNA plasmid fragment pTWIN-1 gene translation amplifier 10 of phage T7, NdeI/BamHI-DNA fragment containing the mini-intein gene Ssp DnaB and sequence of ARNS3 recombinant gene adapted to these sites. The plasmid also contains a b-lactamase gene that determines the resistance of E. coli cells transformed by plasmid pER-ARNS3 to penicillin antibiotics, as a genetic marker and unique restriction endonuclease recognition sites. This invention also discloses a strain of Escherichia coli C3030/pER-ARNS3, producing the said fusion polypeptide. This invention also discloses a method for production of the said ARNS3 recombinant protein, comprising culturing of the said strain of Escherichia coli C3030/pER-ARNS3. n EFFECT: invention provides recombinant ARNS3 with high yield according to a simplified technology. n 3 cl, 4 dwg, 4 ex |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021235983-A1 |
| priorityDate |
2015-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |