| abstract |
FIELD: biochemistry. n SUBSTANCE: present invention relates to biochemistry, in particular to a conjugate of urate oxidase (uricase), intended for decreasing uric acid levels a subject. Said conjugate comprises isolated shortened mammal uricase, having amino acid sequence SEQ ID NO:11, which is shortened from amine end by 6 amino acids and additionally contains amino acid replacements S46T, D7T, R291K and T301S, and polyethylene glycol. Present invention discloses a pharmaceutical composition for reducing uric acid levels in a subject, as well as a method of decreasing uric acid levels in a subject using said conjugate of uricase and polyethylene glycol. n EFFECT: present invention widens the range of products for controlling excess concentration of uric acid (hyperuricemia) in an individual when treating such diseases as gout, gout nodule, renal failure and others. n 45 cl, 8 dwg, 7 tbl, 8 ex |