http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2592860-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_074b3397f09542cb56e796b7ebbde371 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195 |
| filingDate | 2015-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8e3a1b9d057e296f52ed6d6a97037dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dab80ddcb4fb9f674ba965524a7fb285 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb428c16b441d90b5d6d5847a937b303 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d6152db1df56b75927893e0c2ec6947 |
| publicationDate | 2016-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2592860-C2 |
| titleOfInvention | RECOMBINANT PLASMID DNA pER-TB4GyrA-AcSer CODING SERINE ACETYLTRANSFERASE CAPABLE OF in vivo ACETYLATION OF N-TERMINAL SERINE DEACETYL-THYMOSIN β4 AND HYBRID PROTEIN CAPABLE OF AUTOCATALYTIC BREAKDOWN TO FORM HUMAN THYMOSIN β4, STRAIN OF Eschrichia coli C3030/pER-TB4GyrA-AcSer PRODUCER OF SAID PROTEINS AND METHOD OF PRODUCING GENETICALLY ENGINEERED HUMAN THYMOSIN |
| abstract | FIELD: biotechnology. n SUBSTANCE: invention relates to genetic and protein engineering and represents recombinant plasmid DNA pER-TB4GyrA-AcSer, intended for simultaneous biosynthesis of two proteins - hybrid polypeptide, containing amino acid sequence of human thymosin β4 and intein, and enzyme E.coli - serine acetyl transferase. Plasmid has molecular weight 5.07 MDa and consists of BsaBI/NdeI DNA fragment of plasmid pTWIN-1, containing T7 promoter sequence and operator lacO; Ndei/PstI DNA fragment containing a sequence of hybrid protein, including adapted to said sites sequence of recombinant human thymosin β4 gene, sequence of intein Mxe GyrA and a sequence of chitin-binding domain; Psti/NcoI DNA fragment coding reduced Shine-Dalgarno sequence and non-standard as start of codon amino acid valine; NcoI/XhoI DNA fragment containing a sequence of serine N-acetyl transferase; DNA fragment containing genetic marker of β-lactamase gene, determining resistance of transformed by plasmid pER-TA1GyrA-AcSer cells of E.coli to penicillin antibiotics; DNA fragment containing a sequence coding replication start point; DNA fragment coding sequence of lactose repressor LacI. Invention also relates to a strain Escherichia coli C3030/pER-TB4GyrA-AcSer, producing hybrid polypeptide containing amino acid sequence of human thymosin β4 and intein, as well as serine acetyl transferase obtained by transformation of cells of Escherichia coli strain C3030 with recombinant plasmid DNA pER-TB4GyrA-AcSer. n EFFECT: invention enables to obtain recombinant human thymosin beta 4 with high output and using a simple technology. n 3 cl, 2 dwg, 3 ex |
| priorityDate | 2015-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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Total number of triples: 93.