| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9ff587a05b610df0a4268d2fc89b5c92 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-10151
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-10122
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-23
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-00051 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-01 |
| filingDate |
2011-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2016-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_231bc027baa7b816ebc3fd456414b5fc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a17d83ec3acd1ab74bda066f88f63e6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99278c0896c78ee87b3a5011f6ba7f3e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8cdea9feb3fe992bda0fb0c6e71c7d1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c0636fa60f3f9289113e2b863f50a99
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce0e063a0f03a39163c74fcfef509764
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61581d29dd3b9d9fe99689393e8fc38c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f742667d74905a4de5e45bcd76f32c14 |
| publicationDate |
2016-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
RU-2590609-C2 |
| titleOfInvention |
Method of purifying bacteriophages |
| abstract |
FIELD: biotechnology. n SUBSTANCE: present invention relates to a method of purifying bacteriophages. Described method includes: a) culturing a bacterial host strain on an appropriate medium, inoculating said culture with a bacteriophage and obtaining a bacteriophage lysate; b) purifying lysate using affinity chromatography; c) using resulting filtrate to prepare a purified bacteriophage, wherein in stage a) bacterial host strain used consists of bacterial cells containing a sequence encoding a fusion protein containing a foreign polypeptide exhibiting an affinity for a chromatography resin used in stage b) and a polypeptide from among structural phage proteins of bacteriophage present in resulting lysate, and said polypeptide having affinity for chromatographic resin, selected from a group comprising His-tag and GST. n EFFECT: disclosed invention enables single-stage and simultaneously time-efficient purification of phage preparations for therapeutic purposes and ensures preservation of antibacterial activity of bacteriophage in case of displacement of bacteriophage from resin, and in case of proteolytic release. n 5 cl, 10 dwg, 1 tbl, 1 ex |
| priorityDate |
2010-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |