http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2577226-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c835516ade8ef36c702f63be5146764d |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-22 |
| filingDate | 2014-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7a6493344b0e6d91703aca71e3a5708 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7953d307386c6fbfe04a3519b92d6f18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19d4a2bf56f9544d2e883626235967a4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a8865d07c941b929fbefa9c0a042cfce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_118e1d2f7d2d691f2ca0a47f317cd71d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb1ab7384d7eb6b39a3bdda98b2d4af4 |
| publicationDate | 2016-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2577226-C2 |
| titleOfInvention | Methods for making bispecific antibodies against cd3*cd19 in flexybody format in mammalian cells |
| abstract | FIELD: biotechnology. n SUBSTANCE: invention relates to biotechnology. Method for producing a recombinant monoclonal antibody against CD3*CD19 in flexybody format. Constructed recombinant expression plasmid DNA encoding a bispecific antibody against the CD3*CD19. Resulting DNA is transfected with CHO cell strain M, and the resulting cell line is cultured. Cultivation is carried out in a perfusion mode in the presence of feeder additives CB1 to CB5 and 1: 1 ratio, with a volume of additive of 25 % by volume of the nutrient medium. Submitted monoclonal antibody against CD3*CD19 flexybody format having two polypeptide chains non-covalently associated with the amino acid sequence of SEQ ID №1 or non-covalently associated two chain polypeptide having the amino acid sequence of SEQ ID №2, obtained in this way. Also provided a recombinant expression plasmid DNA pOG776, encoding a polypeptide having the sequence bispecific antibody in flexybody format against CD19*CD3, having a size of 10,618 bp, and a recombinant expression plasmid DNA pOG854, encoding a polypeptide with the sequence of the bispecific anti-CD3 * CD19, having a size of 10,618 bp with the physical map shown in fig. 6. n EFFECT: invention provides a high yield of protein of pharmaceutical quality, suitable for the production of drugs. n 6 cl, 8 dwg, 3 tbl, 6 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2791749-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2021505182-A |
| priorityDate | 2014-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.