http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2573930-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ba077e93e17ded206b4fb9b8d5f83f95 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-775 |
| filingDate | 2014-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26071047c5cb96f2394280b54b2cb6a5 |
| publicationDate | 2016-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2573930-C1 |
| titleOfInvention | RECOMBINANT DNA PROVIDING IN COMPOSITION OF PLASMID VECTOR SYNTHESIS IN CELLS OF Ecoli OF CHIMERIC PROTEIN - A-I APOLIPOPROTEIN OF HUMAN BEING |
| abstract | FIELD: biotechnologies. n SUBSTANCE: recombinant DNA is offered, which provides in the composition of a plasmid vector the synthesis in cells of E.coli of a chimeric protein - apolipoprotein A-I of a human being, with the size of 852 bps. The recombinant DNA in series includes from 5'- end DNA fragment coding 6 a.o. of histidine with the size of 18 bps (SEQ ID NO 1), DNA fragment coding 6 a.o. pro-form of the protein apoA-I of a human being with the size of 18 bps (SEQ ID NO 2), DNA fragment coding N-end fragment from 15 a.o. of bull pancreatic RNAase A (SEQ ID NO 3) with the size of 45 bps, DNA fragment coding 6 a.o. pro-form- of the protein apoA-I of a human being with the size of 18 bps (SEQ ID NO 2), DNA fragment coding the site of the recognition of protease of a tobacco mosaic virus with the size of 21 bps (SEQ ID NO 4), mutant gene of a mature protein apoA-I of a human being with the size of 729 bps (SEQ ID NO 5). The yield of the chimeric protein of apolipoprotein A-I of a human being from 1 l of the culture of the cells E.coli is 182±32 mg that amounts 39.7±6.2 mg from 1 g of the biomass. n EFFECT: veracity improvement. n 5 dwg, 3 tbl, 9 ex |
| priorityDate | 2014-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 352.