http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2570625-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8bfcf7ab8c21f194b706e2e3fb7891ee |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-145 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-14 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2011-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6fd0d1a7c09ccd7cb6d4aa6f7ead7168 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15d33b6086fdbd40fedb26183c4c3b8d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83c58fd9e07e764bcf7315dd1b1d0674 |
| publicationDate | 2015-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2570625-C2 |
| titleOfInvention | Separation of non-soluble target proteins |
| abstract | FIELD: chemistry. n SUBSTANCE: claimed invention relates to biotechnology. Claimed is method for separation of recombinant non-soluble target silk protein from suspension of intact or destroyed host cells. Claimed method includes obtaining suspension of intact or destroyed host cells, which contain recombinant non-soluble silk protein, addition of base water solution to said suspension to destroy host cells and for solubilisation of non-soluble parts of host cells, in which silk protein remains unsolved in suspension, separation of recombinant non-soluble silk protein from solubilised parts of host cells. Base represents metal or ammonium hydroxyl, and base water solution has pH values from 9 to 14. n EFFECT: claimed separation method makes it possible to obtain recombinant silk protein with high output and purity due to effective separation of non-soluble recombinant silk protein from solubilised non-soluble parts of host cells, with claimed method being fast and easily realised purification method. n 22 cl, 1 tbl, 4 ex, 6 dwg |
| priorityDate | 2010-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 91.