http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2563540-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_34f094670dacfc1c70350b9e58482e3f |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2013-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f35643538d3505bb00d5d80d66c8af8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8475f7f3528251fcb08b53f93cda6db9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f36076a8b009643efb6d982dc4a6bae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb35ccaa25619bdef85f2248488605fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_909e4e50e9c65469b3f76cd9ad9e6949 |
| publicationDate | 2015-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2563540-C2 |
| titleOfInvention | Method for obtaining recombinant protein sav-rgd, specifically recognising melanoma cells |
| abstract | FIELD: medicine, pharmaceutics. n SUBSTANCE: invention relates to biotechnology. A method of obtaining the recombinant protein SAV-RGD, specifically recognising human melanoma cells, includes cultivation in a nutrient medium of the strain Escherichia coli MG1655/pSAV-RGD, obtaining a periplasmatic fraction of the said strain cells, purification of the recombinant protein by ion-exchange chromatography with the further ultrafiltration and lyophilisation. The strain E. coli MG1655/pSAV-RGD is obtained by the transformation of the strain E. coli MG1655 with the plasmid pSAV-RGD, obtained on the basis of the vector pUC18 and including a sequence, coding the fused pro-protein SAV-RGD (pro-sav-rgd) under the control of a promoter of uridine phosphorylase (Pudp) E. coli. In the process of cultivation feeding with a 40% solution of glucose is realised for the first 10 hours at the feeding rate of 25 ml/hour and supporting glucose at the level of 1 g/l, sterile peptone solution 10 g/l, yeast extract 5 g/l and ampicilline 10 mg/l are fractionally added with the continuation of cultivation for 8 hours. The periplasmatic fraction of cells is obtained by the addition to the cell suspension of a lysing buffer, containing lysozyme in the concentration of 1 g/l, with the ratio of volumes of the lysing buffer and the cell suspension of 1:20. n EFFECT: invention makes it possible to obtain the target protein with high purity, constituting not less than 97%. n 2 cl, 15 dwg, 2 tbl, 8 ex |
| priorityDate | 2013-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 90.