http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2560588-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_753f4737e07b8a621c559df730f1d910 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
| filingDate | 2014-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91f3e43578291bd1a6917d0598aa05ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_283bb5f10e5c22704c1d9095f50e88cf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03590cada9342c96aa9b0e8eda87321b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_222ccbee05df8672475a2f83ed95cb30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5a05b87e77afcb8d5332aec47625f1b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_945c7fd90f54c0aa990772d19388f806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93bfa965c49d810ffa64c27e44107cbb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2d91726a34b31e2ccf4fc1df40e58ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3869c0a1a2a9b302860b82898510edf8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4156ebe2c26a189408488d890699c268 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f4885fbcd35051ddca233a0f756c1c8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc2ab356bba180e630f30c4b9c11d042 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b2077864bc42f5939526d3112649ce4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7186d6aaea08c74facab0e0aba19dd6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50cc982d4394cbe2bc5be5008db0ff04 |
| publicationDate | 2015-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2560588-C1 |
| titleOfInvention | METHOD OF PREPARING IMMUNOGENIC COMPOSITION BASED ON THREE FUSION PROTEINS OF SHELL OF TICK-BORNE ENCEPHALITIS VIRUS DEFINING BELONGING TO SIBERIAN (DBD2-D3S), EUROPEAN (DBD2-D3E) AND FAR EAST (DBD2-D3D) VIRUS SUBTYPES; RECOMBINANT PLASMIDS pDBD2-D3S, pDBD2-D3E AND pDBD2-D3D; PRODUCER STRAINS Escherichia coli M15 [pREP4]; CHIMERIC PROTEINS AND THEIR APPLICATION |
| abstract | FIELD: biotechnology.SUBSTANCE: production of synthetic genes D3S, D3E and D3D is described, optimised for heterologous expression in non-pathogenic laboratory strains of Escherichia coli. These genes encode the receptor-binding domains III of protein E of the shell of tick-borne encephalitis virus (TBEV). On the basis of genes D3S, D3E and D3D the recombinant plasmids pDBD2-D3S, pDBD2-D3E and pDBD2-D3D are received, which encode the bifunctional recombinant proteins which differ in amino acid composition in positions 166, 170, 184, 211 and determining belonging to three main genetic types of the virus. The invention also comprises the producer strains of the chimeric proteins of E. coli M15 [pREP4, pDBD2-D3S], E. coli M15 [pREP4, pDBD2-D3E] and E. coli M15 [pREP4, pDBD2-D3D], and also a method of immobilisation, concentration and purification of the proteins obtained on dextran-containing sorbent. The invention relates to recombinant proteins DBD2-D3S, DBD2-D3E and DBD2-D3D, intended for use as antigens immobilised in the wells of 96-well plate or strips, as part of a set of diagnostic markers for detection of antibodies to TBEV in blood serum and human cerebrospinal fluid by EIA, as well as to an immunogenic composition comprising recombinant proteins immobilised on dextran and directed to the specific activation of immunity and the formation of immunological memory against the virus.EFFECT: invention enables to obtain producer strains providing high level of production of recombinant protein antigens DBD2-D3S, DBD2-D3E and DBD2-D3D, for subsequent use for TBEV immunoprophylaxis, the differential diagnosis of flavivirus infections and assessment of immunity stress.14 cl, 7 dwg |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112899241-A |
| priorityDate | 2014-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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