http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2549710-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e3687df8b5019fe5315e7a90cb08c697 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K2-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-02 |
| filingDate | 2012-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ba10bbfd3f53c95ca3218fb2d839b82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c10119bbe5ff718dae0cc6913205e0d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_826fa76ed14d27f427fc44946ca29c1e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1621bba170be2411fe6603d08091b440 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3701a2a89aef226016533d9ebec9e50c |
| publicationDate | 2015-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2549710-C2 |
| titleOfInvention | Method for purifying recombinant protein of type iii interferon-like factor |
| abstract | FIELD: medicine, pharmaceutics. n SUBSTANCE: invention refers to biotechnology. What is presented is a method for preparing a recombinant protein of type III interferon-like factor (ILF III) of the producing strain E. coli. The inclusion bodies E. coli are washed and dissolved with using 2% aqueous γ-cyclodextrin. That is followed by the sequential Ni-Sepharose, Q-Sepharose and SP-Sepharose chromatographic procedures. Refolding of a target protein is performed with using a mixture of cysteamine and cystamine at pH 10.5. The Amberchrome Profile XT20, Amberchrome Profile HPR10 and Kromasil 300-5C18 chromatographic procedures are sequentially performed. n EFFECT: invention enables optimising the ILF III purification environment at the stage of washing and dissolving the inclusion bodies Ecoli and provides 12% target protein yield. n 3 dwg, 4 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2792435-C2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2650755-C1 |
| priorityDate | 2012-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.