http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2541777-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9a8804e9e5c36ac4a02788e579f021a6 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-34 |
| filingDate | 2013-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e591ea854b4a99016857387ab1ab92d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51a08d3e1d0a8abd4697c2057b2a9a13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2d139ad7844a1b5678428d3907101b5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab487262fd8d92a8465a05e56953487d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d147c31b1b31e4e9cbc27581b2459681 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5719a09ec5d9f5352627cb3b4d7e7eda http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ddc2f1063ae68c5c12d42330c2875be http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec66827258b0284fbc62ef0c21a317b1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0b8cd155853c8b561438841638d1d54 |
| publicationDate | 2015-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2541777-C1 |
| titleOfInvention | RECOMBINANT PLASMID DNA pcDNA4-Apo-2NLS2 CARRYING SYNTHETIC GENE OF APOPTIN PROTEIN INDUCING p53-INDEPENDENT PROGRAMMED TUMOUR CELL DEATH |
| abstract | FIELD: medicine, pharmaceutics. n SUBSTANCE: invention refers to molecular biology, more specifically to recombinant expression of apoptin, and can be used as an anti-tumour therapeutic transgene. The engineered plasmid DNA pcDNA4-Apo-2NLS2 has a molecular weight of 5.13 mD, a size of 7,896 base pairs and contains in accordance with a physical and genetic map presented in Fig. 5, an operon having a size of 1,071 base pairs consisting of the early late promoter CMV, a leader fragment, a sequence of the penetrating peptide of TAT HIV protein, an apoptin gene of chick anaemia virus and a sequence of Flag epitope; the promoter CMV of 232-819 base pairs; T7 promoter of 863-882 base pairs; Xpres primer of 1,112-1,130 base pairs; TOPO cloning site of 1,184 base pairs; genetic markers: the resistance gene Zeocin; b-lactamase gene determining a resistance to ampicillin; unique recognition sites: ClaI (1918); XbaI (2619); BamHI (2993); SalI (3001); BstWI (3425). The produced plasmid DNA pcDNA4-Apo-2NLS2 applied for the apoptin protein expression with the double site NLS2 selectively inducing the p53-independent programmed death of individual's neoplastic tumour cells. The synthetic apoptin gene as a component of the above DNA is characterised by a nucleotide sequence SEQ ID NO1 and a size of 393 base pairs. n EFFECT: invention enables providing higher activity of the expressed modified apoptin protein gene in relation to inducing the selective programmed tumour cell death. n 9 dwg, 1 tbl, 2 ex |
| priorityDate | 2013-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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Total number of triples: 58.