| titleOfInvention |
RECOMBINANT PLASMID pESAT6-CFP10-DBD, RECOMBINANT STRAIN Escherichia coli M15 [pREP4, pESAT6-CFP10-DBD], METHOD FOR PREPARING, IMMOBILISING, CONCENTRATING AND PURIFYING RECOMBINANT PROTEIN ESAT6-CFP10-DBD ON DEXTRAN, RECOMBINANT PROTEIN ESAT6-CFP10-DBD AND IMMUNOGENIC COMPOSITION CONTAINING PROTEIN ESAT6-CFP10-DBD |
| abstract |
FIELD: medicine, pharmaceutics. n SUBSTANCE: group of inventions refers to gene engineering, biochemistry, biotechnology and immunology, and represents recombinant plasmid pESAT6-CFP10-DBD, recombinant strain Escherichia coli M15 [pREP4, pESAT6-CFP10-DBD], a method for preparing, immobilising, concentrating and purifying recombinant protein ESAT6-CFP10-DBD on dextran, recombinant protein ESAT6-CFP10-DBD with molecular weight 26.4 kDa, an immunogenic composition containing protein ESAT6-CFP10-DBD immobilised on dextran, specifically activating T-lymphocytes synthesising IFN-gamma with antigen stimulation of mycobacteria. n EFFECT: invention enables ensuring the high level of protein expression, which induces the immune response to mycobacterial antigen effectively. n 5 cl, 2 dwg, 1 tbl, 5 ex |