http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2525712-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c755d033e86a34a501fc6b32f10fdb6d |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2012-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a992caafa9ed8fa35e65235e350d00e4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ff84a11d7d0960fcbc04d2d70288861 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d2c5e471301c3f31aa58bab8d1f2a49 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06aa6ef921977deb2df5440f6beb28c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b43f69b7dc6f0cfec6a4b706c381f95 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e2bae5bb11a5032c1e7cd397cadf5d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4fba19943ca3ddbcd2b03d6bb406ef7 |
| publicationDate | 2014-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2525712-C2 |
| titleOfInvention | Method for creating transgenic animals with stable and high target protein expression in milk |
| abstract | FIELD: medicine. n SUBSTANCE: invention refers to biotechnology, particularly to methods for preparing next generation drug preparations and biologically active additives in bioreactors on the basis of transgenic producing mammals. The method for creating transgenic animals producing a protein with stable and high expression in milk, involves producing transgenic mammals with using a vector containing a reporter gene coding a target protein that is a goat beta-casein gene promoter, a bovine growth factor terminator and effective two-fold transcription terminators. The terminators surround an expression cartridge and possess an ability to break genome transcripts effectively in a mammalian genome by the effective protection of transgene expression in the mammalian genome against further repression. The effective two-fold terminators represent any mammalian genome site fulfilling the following conditions: 3'-sites of the two simultaneously expressing and opposite genes containing a site of the second-to-last exon, the last intervening sequence, the last exon and a polyadenylation signal, a space of two polyadenylation signals at different DNA strands is no more than 100 base pairs. n EFFECT: method can be used for creating the transgenic animals with high and stable target protein production in milk for medical and research purposes. n 4 dwg, 4 tbl, 3 ex |
| priorityDate | 2012-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 314.