http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2524115-C2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_66733cda41701992f2a57de05499f6d1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d94efc76747f8314a0b9485e84cb4bd0 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2009-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c5f54ca735276ccd4bdad7528cac6e47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3eb4249c92e8c746bd135983d53860d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_431661e27db6cef9d1b69262a940d3cd |
publicationDate | 2014-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2524115-C2 |
titleOfInvention | Method of specific detection of poorly represented rna fractions in biological sample |
abstract | FIELD: medicine, pharmaceutics. n SUBSTANCE: invention relates to the field of molecular biology and genetic engineering. Claimed are: a method and a set for the detection of poorly represented RNA fractions in a biological sample and for the detection of mycoplasma contamination, for which purpose used are operations of reverse transcription of RNA into cDNA with the application of reverse transcriptase Thermus thermophilus (rTth-pol) under conditions of "hot start", RT inactivation by processing with a chaotropic preparation, detection of cDNA of interest by a polymerase chain reaction (PCR). n EFFECT: invention can be used in medicine in testing for the mycoplasma presence. n 15 cl, 3 tbl, 3 ex |
priorityDate | 2008-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 75.